Jim,
See my post above re: Apivar--What went wrong? I sampled my hives and found three that were above my threshold to treat. After treatment I sampled again. Two of the hives showed one or zero mites. But one had gone from seven mites to 43 mites. If I hadn't retested, that hive would have been overwhelmed with mites and died. I wrote to a Master Beekeeper about this failure. Here's part of her reply:
"Good for you for checking on your mite counts after a treatment - I recommend that all the time, but I think I get few takers as it probably sounds like busy work. But your case proves my point: every now and then there is a treatment "failure", or at least, the treatment appears to have been ineffective. And not knowing that fact can leave a beekeeper with a false sense of security."